| 网站首页 | 眼科中心 | 协和眼科 | 会议中心 | 医学资源 | 精美图片 | 论坛 | 会员中心 | 帮助中心 | 换眼科点 | 申请权限 | 全站搜索 | 博客 | 手术视频 | 眼科淘宝 | 
最新公告:

  没有公告

您现在的位置: 心灵之窗-眼科医生网 >> 会议中心 >> 第13届全国会 >> 组织细胞工程13 >> 正文
专题栏目
更多内容
相关文章
更多内容
Geldanamycin, a HSP90 inhibitor, attenuates the hypoxia-induced vascular endothelial growth factor expression in pigment epithelium cells in vitro         
Geldanamycin, a HSP90 inhibitor, attenuates the hypoxia-induced vascular endothelial growth factor expression in pigment epithelium cells in vitro
作者:Wen-Chua… 文章来源:Departments of Ophthalmology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan 点击数:5995 更新时间:2008/5/21 9:49:27
Abstract Hypoxia is the most common factor contributing to the pathogenesis of choroidal neovascularization, which is the major cause for blindness and occurs in proliferative diabetic retinopathy and age-related macular degeneration. The purpose of this study is to investigate the role of retinal pigment epithelial (RPE) cells in the regulation of subretinal neovascularization under hypoxia and the possible function of a heat shock protein 90 (HSP90) inhibitor, geldanamycin (GA), in the regulation of VEGF expression. An in vitro hypoxic experimental model was used to mimic the ischemic microenvironment of RPE cells. The cell growth was measured by proliferation assay and the morphological observation was documented by microscope. The gene expression of VEGF, HSP70, HSP90?and HSP90? were measured using semi-quantitative RT-PCR. The VEGF release from RPE cells were detected by ELISA. No alteration in growth rate and cell morphology under 1% O2 condition for 24h was noticed. The proangiogenic growth factor VEGF, but not bFGF, released from hypoxia-treated cells were higher than that of normoxic control. A similar tendency of VEGF gene expression, detected by RT-PCR, was noticed in hypoxia-treated cells. Heat shock pretreatment elevated HSP70 and VEGF gene expression and augmented the hypoxia-induced VEGF gene expression and protein release. Pretreatment with GA can significantly suppress the hypoxia-induced VEGF gene expression in and peptide release from RPE cells. This in vitro finding suggests that HSP90 inhibitors could be considered as a novel anti-angiogenesis agent for diseases with intraocular neovascularization.
会议投稿录入:毛进    责任编辑:毛进 
  • 上一篇会议投稿: 没有了

  • 下一篇会议投稿:
  • 【字体: 】【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口
      网友评论:(只显示最新10条。评论内容只代表网友观点,与本站立场无关!)

    | 设为首页 | 加入收藏 | 联系站长 | 友情链接 | 版权申明 |
    眼科医生网 眼科医生网版权所有 @ 1998-2012
    部分文章和资源来源于网络,如果侵犯了您的版权,请指出! 站长:毛进
    信息产业部备案
    *京ICP备12035550号